Korrosionsverhalten von Schweißverbindungen des Verbundwerkstoffes CuNiFe/Stahl – Teil 1: Kurzzeit- und Meerwasserloop-Versuche

Corrosion properties of welded CuNiFe/steel-compounds–Part 1: Short time and ASTM-seawater loop tests Short time corrosion tests and ASTM-seawater loop tests at 90°C were performed on CuNi10Fe roll-clad steel compound joint weldings and on surface, spot and repair weldings in order to examine the effect of iron pickup from the base metal (C-steel) or form base layers. In comparison CuNi10-sheets with different Fe-contents and TIG-weldings on these sheets were tested. In ASTM-seawater the corrosion resistance of welding is not altered for iron dilutions with integral values between 1.5 and 5%. Iron concentrations lower than 1.5% result in a reduction of the corrosion resistance. Selective corrosion at the heat affected zone was not observed. In second part of this investigation deals with the corrosion properties in seawater (Helgoland) [13].     

AN ANALYSIS OF STRESS FIELDS OCCURRING IN FLUIDISED BEDS OF PARTICLES WITH PARTICULAR REFERENCE TO SLUGGING SYSTEMS

The behaviour of the dense phase of a fluidised bed is analysed using classical soils mechanics theory. or square nosed slugging it is shown that the plugs of particles separating gas slugs are in the critical state of compaction and the length of these plugs can be predicted from the physical properties of the system     

Nonwetting flow of a liquid through a packed bed with gas cross-flow

In this study, the effect of a cross-flow gas field on the percolating flow of a non-wetting liquid through a packed bed was investigated. Experiments were conducted to measure the liquid shift, due to the cross-flow of air, for the flow of aqueous barium chloride solutions and mercury percolating through beds of polyethylene and expanded polystyrene particles. An X-ray technique was used to visualize the liquid flow pattern through the packed bed. The liquid percolates through a packed bed as a series of rivulets and droplets which are continuously breaking up and coalescing. A mathematical model to predict the direction of the liquid rivulet/droplet flow under the influence of a gas flow field was developed. The model treats the liquid as a discrete phase and includes the effects of gravity, gas drag, and inertial and viscous bed resistance. The effective droplet/rivulet size is an important model parameter, and the model postulates that the droplet/rivulet size is a function of both the effective capillary size of the bed and the liquid flow rate. A simplified population balance analysis for droplet coalescence is used to predict the effect of liquid flow rate on droplet/rivulet size. The model predictions are consistent with the experiments.     

Neuropilin-1 is a placenta growth factor-2 receptor

Placenta growth factor (PlGF) belongs to the family of vascular endothelial growth factors (VEGFs). It binds to the flt-1 VEGF receptor but not to the KDR/flk-1 receptor which is thought to mediate most of the angiogenic and proliferative effects of VEGF. Three PlGF isoforms are produced by alternative splicing. PlGF-1 and PlGF-3 differ from PlGF-2 since they lack the exon 6 encoded peptide which bestows upon PlGF-2 its heparin binding properties. Cross-linking experiments revealed that 125I-PlGF-2 binds to two endothelial cell surface receptors in a heparin dependent fashion. The binding of 125I-PlGF-2 to these receptors was inhibited by an excess of PlGF-2 and by the 165-amino acid form of VEGF (VEGF165), but not at all by VEGF121 and very marginally if at all by PlGF-1. The apparent molecular weight and the binding characteristics of these receptors correspond to those of the recently identified VEGF165 specific receptor neuropilin-1, and we therefore conclude that neuropilin-1 is a receptor for PlGF-2. The binding of 125I-PlGF-2 as well as the binding of 125I-VEGF165 to these receptors was inhibited by a synthetic peptide derived from exon 6 of PlGF. Furthermore, the binding of 125I-PlGF-2, but not that of 125I-VEGF165, was also inhibited by a synthetic peptide derived from exon 7 of PlGF. These observations indicate that the peptides encoded by these exons probably participate in the formation of the domain which mediates the binding of PlGF-2 to these receptors. We have also determined, using chemically modified heparin species, that the presence of sulfate moieties on the glucosamine-O-6 and on the iduronic acid-O-2 groups of heparin was required for the potentiation of 125I-PlGF-2 binding to these receptors. To determine if PlGF-2 is able to induce biological responses that are not induced by PlGF-1, we compared the effects of PlGF-1 and PlGF-2 on the migration and proliferation of endothelial cells. Both PlGF forms induced migration of endothelial cells. However, there was no quantitative difference between the response to PlGF-2 and the response to PlGF-1. Furthermore, neither PlGF-1 nor PlGF-2 had any effect upon the proliferation of the endothelial cells.     

Advances in refolding of proteins produced in E. coli

Guanabenz (Wytensin) was shown to inactivate nitric oxide synthase (NOS) activity in vitro and in vivo. In in vitro studies with the use of a cytosolic fraction from penile tissue, the inactivation was found to depend on NADPH, time, and the concentration of guanabenz. The L-, but not the D-, isomer of arginine could protect from the inactivation, suggesting an active site-directed event. The kinetics of inactivation could be described by an apparent dissociation constant for the initial reversible complex (Ki) and a pseudo first-order inactivation constant (kinact) of 38.5 microM and 0.179 min-1, respectively. In in vivo studies, guanabenz was shown to inhibit penile cytosolic NOS activity in a dose- and time-dependent manner. Treatment of rats with guanabenz (5 mg/kg/day) for 4 days caused a decrease of approximately one-half in the NOS activity of the penile cytosolic fraction with a concomitant loss in the amount of immunodetectable NOS protein. Treatment for 4 days at a dose of 0. 5 mg/kg/day showed a similar decrease in activity, whereas a dose of 0.05 mg/kg/day showed no effects. Due to the multitude of processes that are regulated by NO, the inactivation of NOS is a potential mechanism to be considered in a variety of biological effects associated with drugs.     

CrFK feline kidney cells produce an RD114-like endogenous virus that can package murine leukemia virus-based vectors

The feline kidney cell line CrFK is used extensively for viral infectivity assays and for study of the biology of various retroviruses and derived vectors. We demonstrate the production of an endogenous, RD114-like, infectious retrovirus from CrFK cells. This virus also is shown to efficiently package Moloney murine leukemia virus vectors.